Virus:Article Title: Prefrontal Cortex Corticotropin-Releasing Factor Neurons Control Behavioral Style Selection under Challenging Situations.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CRF Bachem Cat# T-4037; RRID: AB_2314240 Mouse anti-Somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse anti-Parvalbumin Millipore Cat# MAB 1572; RRID: AB_2174013 Mouse anti-Vasoactive intestinal peptide Santa Cruz Cat# sc-25347; RRID: AB_628439 Mouse anti-Calretinin Millipore Cat# MAB 1568; RRID: AB_94259 Rabbit anti-Calbindin Cell Signaling Cat# 13176; RRID: AB_2687400 Alexa Fluor 488-conjugated donkey anti-rabbit Jackson ImmunoResearch Cat# 711-545-152; RRID: AB_2313584 Alexa Fluor 488-conjugated donkey anti-mouse Jackson ImmunoResearch Cat# 715-545-151; RRID: AB_2341099 Bacterial and Virus Strains AAV-DIO-ChR2(H134R)-mCherry BrainVTA Wuhan Cat# PT-0002 AAV-DIO-eNpHR3.0-mCherry BrainVTA Wuhan Cat# PT-0007 AAV-DIO-GCaMP6f Obio Technology Shanghai N/A AAV-DIO-taCasp3-TEVp Taitool Bioscience Shanghai Cat# S0236-9 AAV-DIO-hM4Di-mCherry Obio Technology Shanghai N/A AAV-DIO-hM3Dq-mCherry BrainVTA Wuhan Cat# PT-0042 AAV-DIO-mCherry BrainVTA Wuhan Cat# PT-0013 Chemicals, Peptides, and Recombinant Proteins Neurobitin 488 Vector laboratories Cat# SP-1125 Kynurenic acid Sigma-Aldrich Cat# K3375 Bicuculline Sigma-Aldrich Cat# 14340 Clozapine N-oxide Sigma-Aldrich Cat# C0832 NueroTrace 435/455 Blue Fluorescent Nissl Stain Thermo Fisher Scientific Cat# N21479 40,6-diamidino-2-phylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 Experimental Models: Organisms/Strains Mouse / C57BL/6N Vital River Cat# 213 Mouse / CD-1(ICR)IGS Vital River Cat# 201 Mouse / Crh-IRES-Cre Jackson laboratory RRID: IMSR_JAX:012704 Mouse / Ai32 Jackson laboratory RRID: IMSR_JAX:012569 Mouse / Ai14 Dr. Minmin Luo NIBS RRID: IMSR_JAX:007914 Mouse / GAD67-GFP Tamamaki et al., 2003 Dr. Fuqiang Xu, WIPM N/A Software and Algorithms IMARIS OXFORD Instrument https://imaris.oxinst.com/ GraphPad Prism GraphPad https://www.graphpad.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/index.html MATLAB R2019a MathWorks https://www.mathworks.com/downloads/ web_downloads/ (Continued on next page) e1 Neuron 106, 1–15.e1–e7, April 22, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER SPSS 17.0 IBM https://www.ibm.com/analytics/ spss-statistics-software Clampfit 10.3 Molecular Devices http://mdc.custhelp.com/app/ answers/detail/a_id/18779 EthoVision XT 8.5 Noulds https://www.noldus.com/ethovision-xth Amira Thermo Fisher Scientific https://www.thermofisher.com/us/en/ home/industrial/electron-microscopy/ electron-microscopy-instrumentsworkflow-solutions/3d-visualizationanalysis-software/amira-life-sciencesbiomedical.html CNMF-E MATLAB toolbox Zhou et al., 2018 https://github.com/zhoupc/ eLife_submission NoRMCorre MATLAB toolbox Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/ NoRMCorre Other 473nm blue laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html 589nm yellow laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html Optical fiber Thorlabs Cat# FT200UMT GRIN lens Go!Foton Cat# ILW-100-P0460-055-NC Miniature microscope for calcium imaging in vivo Barbera et al., 2016 https://github.com/giovannibarbera/ miniscope_v1.0 Automated surgical instrument for GRIN lens implantation Liang et al., 2018 https://github.com/liang-bo/AutoStereota
Bioprocessing:Article Title: Prefrontal Cortex Corticotropin-Releasing Factor Neurons Control Behavioral Style Selection under Challenging Situations.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CRF Bachem Cat# T-4037; RRID: AB_2314240 Mouse anti-Somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse anti-Parvalbumin Millipore Cat# MAB 1572; RRID: AB_2174013 Mouse anti-Vasoactive intestinal peptide Santa Cruz Cat# sc-25347; RRID: AB_628439 Mouse anti-Calretinin Millipore Cat# MAB 1568; RRID: AB_94259 Rabbit anti-Calbindin Cell Signaling Cat# 13176; RRID: AB_2687400 Alexa Fluor 488-conjugated donkey anti-rabbit Jackson ImmunoResearch Cat# 711-545-152; RRID: AB_2313584 Alexa Fluor 488-conjugated donkey anti-mouse Jackson ImmunoResearch Cat# 715-545-151; RRID: AB_2341099 Bacterial and Virus Strains AAV-DIO-ChR2(H134R)-mCherry BrainVTA Wuhan Cat# PT-0002 AAV-DIO-eNpHR3.0-mCherry BrainVTA Wuhan Cat# PT-0007 AAV-DIO-GCaMP6f Obio Technology Shanghai N/A AAV-DIO-taCasp3-TEVp Taitool Bioscience Shanghai Cat# S0236-9 AAV-DIO-hM4Di-mCherry Obio Technology Shanghai N/A AAV-DIO-hM3Dq-mCherry BrainVTA Wuhan Cat# PT-0042 AAV-DIO-mCherry BrainVTA Wuhan Cat# PT-0013 Chemicals, Peptides, and Recombinant Proteins Neurobitin 488 Vector laboratories Cat# SP-1125 Kynurenic acid Sigma-Aldrich Cat# K3375 Bicuculline Sigma-Aldrich Cat# 14340 Clozapine N-oxide Sigma-Aldrich Cat# C0832 NueroTrace 435/455 Blue Fluorescent Nissl Stain Thermo Fisher Scientific Cat# N21479 40,6-diamidino-2-phylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 Experimental Models: Organisms/Strains Mouse / C57BL/6N Vital River Cat# 213 Mouse / CD-1(ICR)IGS Vital River Cat# 201 Mouse / Crh-IRES-Cre Jackson laboratory RRID: IMSR_JAX:012704 Mouse / Ai32 Jackson laboratory RRID: IMSR_JAX:012569 Mouse / Ai14 Dr. Minmin Luo NIBS RRID: IMSR_JAX:007914 Mouse / GAD67-GFP Tamamaki et al., 2003 Dr. Fuqiang Xu, WIPM N/A Software and Algorithms IMARIS OXFORD Instrument https://imaris.oxinst.com/ GraphPad Prism GraphPad https://www.graphpad.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/index.html MATLAB R2019a MathWorks https://www.mathworks.com/downloads/ web_downloads/ (Continued on next page) e1 Neuron 106, 1–15.e1–e7, April 22, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER SPSS 17.0 IBM https://www.ibm.com/analytics/ spss-statistics-software Clampfit 10.3 Molecular Devices http://mdc.custhelp.com/app/ answers/detail/a_id/18779 EthoVision XT 8.5 Noulds https://www.noldus.com/ethovision-xth Amira Thermo Fisher Scientific https://www.thermofisher.com/us/en/ home/industrial/electron-microscopy/ electron-microscopy-instrumentsworkflow-solutions/3d-visualizationanalysis-software/amira-life-sciencesbiomedical.html CNMF-E MATLAB toolbox Zhou et al., 2018 https://github.com/zhoupc/ eLife_submission NoRMCorre MATLAB toolbox Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/ NoRMCorre Other 473nm blue laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html 589nm yellow laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html Optical fiber Thorlabs Cat# FT200UMT GRIN lens Go!Foton Cat# ILW-100-P0460-055-NC Miniature microscope for calcium imaging in vivo Barbera et al., 2016 https://github.com/giovannibarbera/ miniscope_v1.0 Automated surgical instrument for GRIN lens implantation Liang et al., 2018 https://github.com/liang-bo/AutoStereota
Recombinant:Article Title: Prefrontal Cortex Corticotropin-Releasing Factor Neurons Control Behavioral Style Selection under Challenging Situations.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CRF Bachem Cat# T-4037; RRID: AB_2314240 Mouse anti-Somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse anti-Parvalbumin Millipore Cat# MAB 1572; RRID: AB_2174013 Mouse anti-Vasoactive intestinal peptide Santa Cruz Cat# sc-25347; RRID: AB_628439 Mouse anti-Calretinin Millipore Cat# MAB 1568; RRID: AB_94259 Rabbit anti-Calbindin Cell Signaling Cat# 13176; RRID: AB_2687400 Alexa Fluor 488-conjugated donkey anti-rabbit Jackson ImmunoResearch Cat# 711-545-152; RRID: AB_2313584 Alexa Fluor 488-conjugated donkey anti-mouse Jackson ImmunoResearch Cat# 715-545-151; RRID: AB_2341099 Bacterial and Virus Strains AAV-DIO-ChR2(H134R)-mCherry BrainVTA Wuhan Cat# PT-0002 AAV-DIO-eNpHR3.0-mCherry BrainVTA Wuhan Cat# PT-0007 AAV-DIO-GCaMP6f Obio Technology Shanghai N/A AAV-DIO-taCasp3-TEVp Taitool Bioscience Shanghai Cat# S0236-9 AAV-DIO-hM4Di-mCherry Obio Technology Shanghai N/A AAV-DIO-hM3Dq-mCherry BrainVTA Wuhan Cat# PT-0042 AAV-DIO-mCherry BrainVTA Wuhan Cat# PT-0013 Chemicals, Peptides, and Recombinant Proteins Neurobitin 488 Vector laboratories Cat# SP-1125 Kynurenic acid Sigma-Aldrich Cat# K3375 Bicuculline Sigma-Aldrich Cat# 14340 Clozapine N-oxide Sigma-Aldrich Cat# C0832 NueroTrace 435/455 Blue Fluorescent Nissl Stain Thermo Fisher Scientific Cat# N21479 40,6-diamidino-2-phylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 Experimental Models: Organisms/Strains Mouse / C57BL/6N Vital River Cat# 213 Mouse / CD-1(ICR)IGS Vital River Cat# 201 Mouse / Crh-IRES-Cre Jackson laboratory RRID: IMSR_JAX:012704 Mouse / Ai32 Jackson laboratory RRID: IMSR_JAX:012569 Mouse / Ai14 Dr. Minmin Luo NIBS RRID: IMSR_JAX:007914 Mouse / GAD67-GFP Tamamaki et al., 2003 Dr. Fuqiang Xu, WIPM N/A Software and Algorithms IMARIS OXFORD Instrument https://imaris.oxinst.com/ GraphPad Prism GraphPad https://www.graphpad.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/index.html MATLAB R2019a MathWorks https://www.mathworks.com/downloads/ web_downloads/ (Continued on next page) e1 Neuron 106, 1–15.e1–e7, April 22, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER SPSS 17.0 IBM https://www.ibm.com/analytics/ spss-statistics-software Clampfit 10.3 Molecular Devices http://mdc.custhelp.com/app/ answers/detail/a_id/18779 EthoVision XT 8.5 Noulds https://www.noldus.com/ethovision-xth Amira Thermo Fisher Scientific https://www.thermofisher.com/us/en/ home/industrial/electron-microscopy/ electron-microscopy-instrumentsworkflow-solutions/3d-visualizationanalysis-software/amira-life-sciencesbiomedical.html CNMF-E MATLAB toolbox Zhou et al., 2018 https://github.com/zhoupc/ eLife_submission NoRMCorre MATLAB toolbox Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/ NoRMCorre Other 473nm blue laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html 589nm yellow laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html Optical fiber Thorlabs Cat# FT200UMT GRIN lens Go!Foton Cat# ILW-100-P0460-055-NC Miniature microscope for calcium imaging in vivo Barbera et al., 2016 https://github.com/giovannibarbera/ miniscope_v1.0 Automated surgical instrument for GRIN lens implantation Liang et al., 2018 https://github.com/liang-bo/AutoStereota
Staining:Article Title: Prefrontal Cortex Corticotropin-Releasing Factor Neurons Control Behavioral Style Selection under Challenging Situations.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CRF Bachem Cat# T-4037; RRID: AB_2314240 Mouse anti-Somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse anti-Parvalbumin Millipore Cat# MAB 1572; RRID: AB_2174013 Mouse anti-Vasoactive intestinal peptide Santa Cruz Cat# sc-25347; RRID: AB_628439 Mouse anti-Calretinin Millipore Cat# MAB 1568; RRID: AB_94259 Rabbit anti-Calbindin Cell Signaling Cat# 13176; RRID: AB_2687400 Alexa Fluor 488-conjugated donkey anti-rabbit Jackson ImmunoResearch Cat# 711-545-152; RRID: AB_2313584 Alexa Fluor 488-conjugated donkey anti-mouse Jackson ImmunoResearch Cat# 715-545-151; RRID: AB_2341099 Bacterial and Virus Strains AAV-DIO-ChR2(H134R)-mCherry BrainVTA Wuhan Cat# PT-0002 AAV-DIO-eNpHR3.0-mCherry BrainVTA Wuhan Cat# PT-0007 AAV-DIO-GCaMP6f Obio Technology Shanghai N/A AAV-DIO-taCasp3-TEVp Taitool Bioscience Shanghai Cat# S0236-9 AAV-DIO-hM4Di-mCherry Obio Technology Shanghai N/A AAV-DIO-hM3Dq-mCherry BrainVTA Wuhan Cat# PT-0042 AAV-DIO-mCherry BrainVTA Wuhan Cat# PT-0013 Chemicals, Peptides, and Recombinant Proteins Neurobitin 488 Vector laboratories Cat# SP-1125 Kynurenic acid Sigma-Aldrich Cat# K3375 Bicuculline Sigma-Aldrich Cat# 14340 Clozapine N-oxide Sigma-Aldrich Cat# C0832 NueroTrace 435/455 Blue Fluorescent Nissl Stain Thermo Fisher Scientific Cat# N21479 40,6-diamidino-2-phylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 Experimental Models: Organisms/Strains Mouse / C57BL/6N Vital River Cat# 213 Mouse / CD-1(ICR)IGS Vital River Cat# 201 Mouse / Crh-IRES-Cre Jackson laboratory RRID: IMSR_JAX:012704 Mouse / Ai32 Jackson laboratory RRID: IMSR_JAX:012569 Mouse / Ai14 Dr. Minmin Luo NIBS RRID: IMSR_JAX:007914 Mouse / GAD67-GFP Tamamaki et al., 2003 Dr. Fuqiang Xu, WIPM N/A Software and Algorithms IMARIS OXFORD Instrument https://imaris.oxinst.com/ GraphPad Prism GraphPad https://www.graphpad.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/index.html MATLAB R2019a MathWorks https://www.mathworks.com/downloads/ web_downloads/ (Continued on next page) e1 Neuron 106, 1–15.e1–e7, April 22, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER SPSS 17.0 IBM https://www.ibm.com/analytics/ spss-statistics-software Clampfit 10.3 Molecular Devices http://mdc.custhelp.com/app/ answers/detail/a_id/18779 EthoVision XT 8.5 Noulds https://www.noldus.com/ethovision-xth Amira Thermo Fisher Scientific https://www.thermofisher.com/us/en/ home/industrial/electron-microscopy/ electron-microscopy-instrumentsworkflow-solutions/3d-visualizationanalysis-software/amira-life-sciencesbiomedical.html CNMF-E MATLAB toolbox Zhou et al., 2018 https://github.com/zhoupc/ eLife_submission NoRMCorre MATLAB toolbox Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/ NoRMCorre Other 473nm blue laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html 589nm yellow laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html Optical fiber Thorlabs Cat# FT200UMT GRIN lens Go!Foton Cat# ILW-100-P0460-055-NC Miniature microscope for calcium imaging in vivo Barbera et al., 2016 https://github.com/giovannibarbera/ miniscope_v1.0 Automated surgical instrument for GRIN lens implantation Liang et al., 2018 https://github.com/liang-bo/AutoStereota
Software:Article Title: Prefrontal Cortex Corticotropin-Releasing Factor Neurons Control Behavioral Style Selection under Challenging Situations.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CRF Bachem Cat# T-4037; RRID: AB_2314240 Mouse anti-Somatostatin Santa Cruz Cat# sc-55565; RRID: AB_831726 Mouse anti-Parvalbumin Millipore Cat# MAB 1572; RRID: AB_2174013 Mouse anti-Vasoactive intestinal peptide Santa Cruz Cat# sc-25347; RRID: AB_628439 Mouse anti-Calretinin Millipore Cat# MAB 1568; RRID: AB_94259 Rabbit anti-Calbindin Cell Signaling Cat# 13176; RRID: AB_2687400 Alexa Fluor 488-conjugated donkey anti-rabbit Jackson ImmunoResearch Cat# 711-545-152; RRID: AB_2313584 Alexa Fluor 488-conjugated donkey anti-mouse Jackson ImmunoResearch Cat# 715-545-151; RRID: AB_2341099 Bacterial and Virus Strains AAV-DIO-ChR2(H134R)-mCherry BrainVTA Wuhan Cat# PT-0002 AAV-DIO-eNpHR3.0-mCherry BrainVTA Wuhan Cat# PT-0007 AAV-DIO-GCaMP6f Obio Technology Shanghai N/A AAV-DIO-taCasp3-TEVp Taitool Bioscience Shanghai Cat# S0236-9 AAV-DIO-hM4Di-mCherry Obio Technology Shanghai N/A AAV-DIO-hM3Dq-mCherry BrainVTA Wuhan Cat# PT-0042 AAV-DIO-mCherry BrainVTA Wuhan Cat# PT-0013 Chemicals, Peptides, and Recombinant Proteins Neurobitin 488 Vector laboratories Cat# SP-1125 Kynurenic acid Sigma-Aldrich Cat# K3375 Bicuculline Sigma-Aldrich Cat# 14340 Clozapine N-oxide Sigma-Aldrich Cat# C0832 NueroTrace 435/455 Blue Fluorescent Nissl Stain Thermo Fisher Scientific Cat# N21479 40,6-diamidino-2-phylindole, dihydrochloride (DAPI) Thermo Fisher Scientific Cat# D1306 Experimental Models: Organisms/Strains Mouse / C57BL/6N Vital River Cat# 213 Mouse / CD-1(ICR)IGS Vital River Cat# 201 Mouse / Crh-IRES-Cre Jackson laboratory RRID: IMSR_JAX:012704 Mouse / Ai32 Jackson laboratory RRID: IMSR_JAX:012569 Mouse / Ai14 Dr. Minmin Luo NIBS RRID: IMSR_JAX:007914 Mouse / GAD67-GFP Tamamaki et al., 2003 Dr. Fuqiang Xu, WIPM N/A Software and Algorithms IMARIS OXFORD Instrument https://imaris.oxinst.com/ GraphPad Prism GraphPad https://www.graphpad.com/ ImageJ National Institutes of Health https://imagej.nih.gov/ij/index.html MATLAB R2019a MathWorks https://www.mathworks.com/downloads/ web_downloads/ (Continued on next page) e1 Neuron 106, 1–15.e1–e7, April 22, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER SPSS 17.0 IBM https://www.ibm.com/analytics/ spss-statistics-software Clampfit 10.3 Molecular Devices http://mdc.custhelp.com/app/ answers/detail/a_id/18779 EthoVision XT 8.5 Noulds https://www.noldus.com/ethovision-xth Amira Thermo Fisher Scientific https://www.thermofisher.com/us/en/ home/industrial/electron-microscopy/ electron-microscopy-instrumentsworkflow-solutions/3d-visualizationanalysis-software/amira-life-sciencesbiomedical.html CNMF-E MATLAB toolbox Zhou et al., 2018 https://github.com/zhoupc/ eLife_submission NoRMCorre MATLAB toolbox Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/ NoRMCorre Other 473nm blue laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html 589nm yellow laser Fiblaser http://www.fiblaser.com/ Product-Wavelength%20List.html Optical fiber Thorlabs Cat# FT200UMT GRIN lens Go!Foton Cat# ILW-100-P0460-055-NC Miniature microscope for calcium imaging in vivo Barbera et al., 2016 https://github.com/giovannibarbera/ miniscope_v1.0 Automated surgical instrument for GRIN lens implantation Liang et al., 2018 https://github.com/liang-bo/AutoStereota
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